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normal human colonic epithelial cells ncm460  (ATCC)


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    ATCC normal human colonic epithelial cells ncm460
    Normal Human Colonic Epithelial Cells Ncm460, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+colonic+epithelial+cells+ncm460/pm41469036-43-10-22?v=ATCC
    Average 97 stars, based on 1415 article reviews
    normal human colonic epithelial cells ncm460 - by Bioz Stars, 2026-07
    97/100 stars

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    MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of <t>NCM460</t> cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.
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    MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of <t>NCM460</t> cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.
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    MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of NCM460 cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.

    Journal: The Turkish Journal of Gastroenterology

    Article Title: Matrine Alleviates Oxidative Stress and Inflammation in Colon Cancer by Activating the Nrf2 Pathway

    doi: 10.5152/tjg.2025.24438

    Figure Lengend Snippet: MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of NCM460 cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.

    Article Snippet: Human CC cells HT-29 (BFN60800646) and human normal colon epithelial cells NCM460 (BFN608006385) were obtained from the American Type Culture Collection (USA).

    Techniques: Cell Counting, Transfection, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control