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normal human colonic epithelial cells ncm460  (ATCC)


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    ATCC normal human colonic epithelial cells ncm460
    Normal Human Colonic Epithelial Cells Ncm460, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+colonic+epithelial+cells+ncm460/Primary+Small+Airway+Epithelial+Cells%3B+Normal%2C+Human/pm41469036-43-10-22
    Average 97 stars, based on 1432 article reviews
    normal human colonic epithelial cells ncm460 - by Bioz Stars, 2026-09
    97/100 stars

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    Knockdown:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Western Blot:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Expressing:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Two Tailed Test:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Activity Assay:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    CCK-8 Assay:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Tube Formation Assay:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Transwell Assay:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Migration:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .

    Control:

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor-Associated Macrophage Infiltration via P4HA2-PI3K-AKT Pathway.
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO2 at 37°C.

    Article Title: Targeting P4HA1 Inhibits Colorectal Cancer Growth, Metastasis, and Tumor‐Associated Macrophage Infiltration via P4HA2‐PI3K‐AKT Pathway
    Article Snippet: The human colorectal adenocarcinoma cell lines HT‐29, SW620, and HCT116, normal human colonic epithelial cells NCM460, and THP‐1 cells were obtained from ATCC.. Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.Cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin‐streptomycin, and cells were maintained under identical conditions in a humidified atmosphere of 5% CO 2 at 37°C.

    Article Title: Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L)
    Article Snippet: Human CRC cell lines, SW480, SW620, HT-29 and HCT116 and normal human colonic epithelial cells NCM460 were obtained from American Type Culture Collection (ATCC, USA).. CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .CRC cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) at 37°C with 5% CO 2 .



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    human normal colon epithelial cells ncm460  (Procell Inc)
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    Procell Inc human normal colon epithelial cells ncm460
    MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of <t>NCM460</t> cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.
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    MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of NCM460 cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.

    Journal: The Turkish Journal of Gastroenterology

    Article Title: Matrine Alleviates Oxidative Stress and Inflammation in Colon Cancer by Activating the Nrf2 Pathway

    doi: 10.5152/tjg.2025.24438

    Figure Lengend Snippet: MAT inhibited viability, oxidative stress, and inflammation of colon cancer cells by activating the Nrf2 pathway. (A) Cell counting kit-8 assay was used to detect the cell viability of NCM460 cells and HT-29 cells. n = 6. (B) The transfection efficiency of si-Nrf2 was confirmed by qPCR. n = 3. (C) Methylthiazolyldiphenyl-tetrazolium bromide assay was used to measure HT-29 cell viability. n = 6. (D) Flow cytometry analysis was employed to test the apoptosis of HT-29 cells. n = 3. (E) The activities of SOD and MDA in HT-29 cells were quantified. n = 6. (F) The levels of IL-6 and TNF-α were tested by ELISA. n = 6. (G) Western blot assays were used to determine the expression levels of Nrf2, KEAP1, NQO1, and HO-1 proteins in HT-29 cells. n = 3. Results were presented as mean ± SD. + P < .05 and ++ P < .01 vs. Control group; # P < .05 and ## P < .01 vs. MAT + si-NC group.

    Article Snippet: Human CC cells HT-29 (BFN60800646) and human normal colon epithelial cells NCM460 (BFN608006385) were obtained from the American Type Culture Collection (USA).

    Techniques: Cell Counting, Transfection, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control